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HER2 expression analysis in spiked SKBR3 BC cell lines. ( A ) IF images of SKBR3 cells and PBMCs recovered from HDB, either spiked with SKBR3 cells (left) or non-spiked (right). Blood was processed using the EpCAM-independent pipeline. Spike-in experiments were conducted in triplicate. The recovered cells were stained for DAPI (blue), <t>CD45</t> (red), EpCAM/CK (green) and HER2 (cyan) markers. The majority of recovered SKBR3 cells were DAPI+, CD45-, EpCAM/CK+, and HER2+, whereas the majority of cells recovered from the non-spiked HDB blood were DAPI+, CD45+, EpCAM/CK-, and HER2- ( B ) Comparative bar chart showing the HER2 expression levels (high, intermediate, and low/none) established on the recovered SKBR3 cells using the EpCAM-independent pipeline.
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HER2 expression analysis in spiked SKBR3 BC cell lines. ( A ) IF images of SKBR3 cells and PBMCs recovered from HDB, either spiked with SKBR3 cells (left) or non-spiked (right). Blood was processed using the EpCAM-independent pipeline. Spike-in experiments were conducted in triplicate. The recovered cells were stained for DAPI (blue), <t>CD45</t> (red), EpCAM/CK (green) and HER2 (cyan) markers. The majority of recovered SKBR3 cells were DAPI+, CD45-, EpCAM/CK+, and HER2+, whereas the majority of cells recovered from the non-spiked HDB blood were DAPI+, CD45+, EpCAM/CK-, and HER2- ( B ) Comparative bar chart showing the HER2 expression levels (high, intermediate, and low/none) established on the recovered SKBR3 cells using the EpCAM-independent pipeline.
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HER2 expression analysis in spiked SKBR3 BC cell lines. ( A ) IF images of SKBR3 cells and PBMCs recovered from HDB, either spiked with SKBR3 cells (left) or non-spiked (right). Blood was processed using the EpCAM-independent pipeline. Spike-in experiments were conducted in triplicate. The recovered cells were stained for DAPI (blue), <t>CD45</t> (red), EpCAM/CK (green) and HER2 (cyan) markers. The majority of recovered SKBR3 cells were DAPI+, CD45-, EpCAM/CK+, and HER2+, whereas the majority of cells recovered from the non-spiked HDB blood were DAPI+, CD45+, EpCAM/CK-, and HER2- ( B ) Comparative bar chart showing the HER2 expression levels (high, intermediate, and low/none) established on the recovered SKBR3 cells using the EpCAM-independent pipeline.
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HER2 expression analysis in spiked SKBR3 BC cell lines. ( A ) IF images of SKBR3 cells and PBMCs recovered from HDB, either spiked with SKBR3 cells (left) or non-spiked (right). Blood was processed using the EpCAM-independent pipeline. Spike-in experiments were conducted in triplicate. The recovered cells were stained for DAPI (blue), <t>CD45</t> (red), EpCAM/CK (green) and HER2 (cyan) markers. The majority of recovered SKBR3 cells were DAPI+, CD45-, EpCAM/CK+, and HER2+, whereas the majority of cells recovered from the non-spiked HDB blood were DAPI+, CD45+, EpCAM/CK-, and HER2- ( B ) Comparative bar chart showing the HER2 expression levels (high, intermediate, and low/none) established on the recovered SKBR3 cells using the EpCAM-independent pipeline.
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HER2 expression analysis in spiked SKBR3 BC cell lines. ( A ) IF images of SKBR3 cells and PBMCs recovered from HDB, either spiked with SKBR3 cells (left) or non-spiked (right). Blood was processed using the EpCAM-independent pipeline. Spike-in experiments were conducted in triplicate. The recovered cells were stained for DAPI (blue), CD45 (red), EpCAM/CK (green) and HER2 (cyan) markers. The majority of recovered SKBR3 cells were DAPI+, CD45-, EpCAM/CK+, and HER2+, whereas the majority of cells recovered from the non-spiked HDB blood were DAPI+, CD45+, EpCAM/CK-, and HER2- ( B ) Comparative bar chart showing the HER2 expression levels (high, intermediate, and low/none) established on the recovered SKBR3 cells using the EpCAM-independent pipeline.

Journal: Cancer Cell International

Article Title: Quantitative HER2 profiling on circulating tumor cells using an EpCAM-independent platform in metastatic breast cancer

doi: 10.1186/s12935-025-04036-x

Figure Lengend Snippet: HER2 expression analysis in spiked SKBR3 BC cell lines. ( A ) IF images of SKBR3 cells and PBMCs recovered from HDB, either spiked with SKBR3 cells (left) or non-spiked (right). Blood was processed using the EpCAM-independent pipeline. Spike-in experiments were conducted in triplicate. The recovered cells were stained for DAPI (blue), CD45 (red), EpCAM/CK (green) and HER2 (cyan) markers. The majority of recovered SKBR3 cells were DAPI+, CD45-, EpCAM/CK+, and HER2+, whereas the majority of cells recovered from the non-spiked HDB blood were DAPI+, CD45+, EpCAM/CK-, and HER2- ( B ) Comparative bar chart showing the HER2 expression levels (high, intermediate, and low/none) established on the recovered SKBR3 cells using the EpCAM-independent pipeline.

Article Snippet: CD45 , Surface , HI30 , Alexa fluor 555 , Bioss Antibodies , bs-0522R-A555 , 1:10 , 30 , TRITC/ Rhodamine.

Techniques: Expressing, Staining